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ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
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Alkaline Phosphatase

Enzyme, Purified from calf intestine

ECO-TEK

Cat. No. 4700

Alkaline Phosphatase (AP) is purified from bovine intestinal mucosa by chromatography and filtration. AP is a zinc metaloenzyme for catalyzing the hydrolysis of an orthophosporic monoester to yield an alcohol and an orthophosphate. AP can be coupled to antibodies resulting in conjugates, used in ELISA, membrane & IHC applications, biosensors etc.

In ELISA, the main colorimetric substrate for AP is p-nitrophenyl phosphate (pNPP) which yields a yellow reaction product. Absorbance can be quantified by reading the OD at 405 nm. The kinetic reaction is terminated by the addition of a stop-solution.

This product is not for sale in the US.

Specifications

  • Available sizes: 10 mg, 100 mg
  • Format: Ready-to-use (RTU)
  • Storage: 2-8⁰C
  • Stability: 1.5 years
  • Activity: > 1500 U/mg enzyme protein (25⁰C, 4-nitrophenyl-phosphate, glycin as buffer).
  • Purity: More than 90% pure (chromatography)
Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
All about eco-tek

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