Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
All about eco-tek

TMB ONE (& prestained)

ELISA HRP Substrate

ECO-TEK

Cat. No. 4380 / 4430

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB ONE characteristics are:

  • “The Gold Standard” used across the industry
  • Incredibly stable and robust
  • Aqueous based – no organic solvents (REACH Annex XVII compliant)
  • Low lot-to-lot variation

For traceable pipetting, we recommend using pink colored TMB ONE Prestained (cat. no. 4430). The pink indicator disappears after addition of the stop solution.

The product is available in bulk quantities and as customized solutions.

Specifications

  • Available sizes: 100 mL, 500 mL, 1 L , bulk
  • Format: Ready-to-use (RTU)
  • Storage: 2-8⁰C in the dark
  • Stability: 4 years
  • Development time: 5-30 minutes
  • Stop solution: 0.2 M H₂SO₄
Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
All about eco-tek

Request a sample on this product

Our products speak for themselves and we would like to share a free sample with you. Please fill out the form: including the product name and/or number that you are interested in trying, and we will contact you regarding the shipping and delivery details of your sample.