Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
All about eco-tek

Horseradish Peroxidase

Enzyme, lyophilized from roots of horseradish

ECO-TEK

Cat. No. 4120

Horseradish Peroxidase (HRP) is derived from the root extracts of the horseradish plant. HRP uses H2O2 to oxidize both organic and inorganic compounds. Antibody conjugates, used in ELISA, membrane & IHC applications, biosensors etc. can be developed when coupling HRP to antibodies. HRP is purified by large scale extraction and purification of horseradish roots by means of ultrafiltration, protein precipitation ion exchange and hydrophobic interaction chromatography, resulting in a highly purified, salt free lyophilized product.

In ELISA, the main colorimetric substrate for HRP is TMB (3, 3', 5, 5'-tetramethylbenzidine). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of an appropriate stop solution gives a clear yellow color that absorbs at 450nm.

Specifications

  • Available sizes: 25 mg, 100 mg, 1 g
  • Format: Lyophilized
  • Storage: Dry at -20 °C or below
  • Stability: 4 years
  • Activity: >250 U/mg (25 °C, Guaiacol as hydrogen donor)
  • Purity: (A403/A275) = RZ > 3.0
Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
All about eco-tek

Request a sample on this product

Our products speak for themselves and we would like to share a free sample with you. Please fill out the form: including the product name and/or number that you are interested in trying, and we will contact you regarding the shipping and delivery details of your sample.