Soluble HRP Substrates

Soluble Horseradish Peroxidase (HRP) Substrates

In a soluble chromogenic HRP assay setup, like an ELISA, the preferred substrate is TMB (3, 3', 5, 5'-tetramethylbenzidine). When developing immunoassays, choosing the right TMB is a vital step for optimizing the assay. Kementec offers a range of TMBs with different kinetics and sensitivities, enabling assay developers to choose the right TMB for their assay.

TMB Kinetics

TMB Kinetics showing 5 of our products

Sensitivity levels and speed of color development of the different TMB formulations shown at incubation time 1-30 minutes.

All the TMBs are ECO-TEK, making them REACH, Annex XVII0 compliant and easy to transport with less restrictions. ECO-TEK includes:

Free of BSA
Free of NMP or other harmful organic solvents – 100% aqueous
Free of toxic preservatives and hazardous stop solutions

The TMBs are characterized by outstanding sensitivity, stability and stop-stability, and very high lot-to-lot consistency. Offering a shelf life up to 4 years of the ready-to-use substrates enables Kementec products to meet all the requirements of assay manufacturers.

Lot-to-lot variation

Lot-to-lot

In order to achieve high quality levels, rigorous QC standards ensure negligible lot-to-lot variations. 50 Lot-to-lot consistency, displayed in the example, shows the last 7 years productions of TMB PLUS2, which lie within a 20% interval.

Stop Solution Benefits

As we focus on environmentally safe products, Kementec substrates require low stop solution concentrations. The superior stop stability maintains the final OD level, as illustrated, where Kementec’s TMB X-tnd is compared to 1 competitor. The stop stability up to 60 minutes drops to no more than 5% in the high OD area.

Several of the TMBs are suitable for use in electrochemical assays that utilize sensor measurements.

TMB substrates are also available as unique color-coded products for traceable pipetting. The pink indicator makes manual pipetting traceable, while in automatic analyzers it offers a convenient tracking system for reagent supply. The color disappears after adding the recommended stop solution and does not interfere with the assay.

Corresponding diluents are available for TMB substrates in order to achieve a specific activity level.

TMB ONE (& prestained)

ELISA HRP Substrate

Available sizes: 100 mL, 500 mL, 1 L , bulk
Format: Ready-to-use (RTU)
Storage: 2-8⁰C in the dark

TMB EASE

ELISA HRP Substrate

Available sizes: 100 mL, 500 mL, 1 L, bulk
Format: Ready-to-use (RTU)
Storage: 2-8⁰C in the dark

TMB PLUS2® (& prestained)

ELISA HRP Substrate

Three brown laboratory bottles in different sizes with Kementec Solutions TMB PLUS2

Available sizes: 100 mL, 500 mL, 1 L, bulk
Format: Ready-to-use (RTU)
Storage: 2-8⁰C in the dark

TMB X-tnd®

ELISA HRP Substrate

Available sizes 100 mL, 500 mL, 1 L, bulk
Format: Ready-to-use (RTU)
Storage: 2-8⁰C in the dark

TMB X-tra (& prestained)

ELISA HRP Substrate

Three brown laboratory bottles in different sizes with Kementec Solutions TMB X-tra

Available sizes: 100 mL, 500 mL, 1 L, bulk
Format: Ready-to-use (RTU)
Storage: 2-8⁰C in the dark