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BCIP/NBT

Membrane AP Substrate

Cat. No. 4410

BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/ Nitro Blue Tetrazolium) liquid ready-to-use substrate is a highly active and stable formulation utilized for colorimetric detection of Alkaline Phosphatase (AP) activity in membrane assays. Positive reactions form an intense blue/purple precipitate at the site of the reaction. The color develops when AP catalyzes the dephosphorylation of BCIP and converts NBT to insoluble blue/purple NBT formazan. The intense blue/purple precipitate is very stable and resists fading when exposed to light.

The product is available in bulk quantities and as customized solutions.

Specifications

  • Available sizes: 100 mL, 500 mL, 1 L, bulk
  • Format: Ready-to-use (RTU)
  • Storage: 2-8⁰C in the dark
  • Stability: 4 years
  • Development time: 5-30 minutes
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