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ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
pNPP ONE
ELISA AP Substrate
ECO-TEK
ANIMAL-FREE
Cat. No. 4401
pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm.
pNPP ONE liquid ready-to-use substrate is active and stable. PNPP ONE shows superior background characteristics due to its high temperature robustness compared to pNPP (cat. no. 4400).
The product is available in bulk quantities and as customized solutions.
Specifications
- Available sizes: 100 mL, 500 mL, 1 L, bulk
- Format: Ready-to-use (RTU)
- Storage: 2-8⁰C in the dark
- Stability: 3 years
- Development time: 5-30 minutes
- Stop Solution: 0,1 M NaOH
Downloads
Download your LOT specific Certificate of Analysis (CoA)
Request a sample
Request
Questions, or buy this product?
Contact us
ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
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