Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
All about eco-tek

pNPP

ELISA AP Substrate

ECO-TEK
ANIMAL-FREE

Cat. No. 4400

pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to p-nitrophenol and inorganic phosphate. Liberated p-nitrophenol is seen as a clear yellow color that is measured at 405/620 nm. pNPP liquid ready-to-use substrate is highly active compared to pNPP ONE (cat. No. 4401). The substrate gives superior results due to its sensitivity, allowing very low detection limits.

The product is available in bulk quantities and as customized solutions.

Specifications

  • Available sizes: 100 mL, 500 mL, 1 L, bulk
  • Format: Ready-to-use (RTU)
  • Storage: 2-8⁰C in the dark
  • Stability: 2 years
  • Development time: 5-30 minutes
  • Stop Solution: 0,1 M NaOH
Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
All about eco-tek

Request a sample on this product

Our products speak for themselves and we would like to share a free sample with you. Please fill out the form: including the product name and/or number that you are interested in trying, and we will contact you regarding the shipping and delivery details of your sample.