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ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
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TMB X-tra (& prestained)

ELISA HRP Substrate

ECO-TEK

Cat. No. 4800 / 4810

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB X-tra characteristics are:

  • High sensitivity
  • Stable and robust
  • Aqueous based – no organic solvents (REACH Annex XVII compliant)
  • Low lot-to-lot variation

For traceable pipetting, we recommend using pink colored TMB X-tra Prestained (cat. no. 4810). The pink indicator disappears after addition of the stop solution.

The product is available in bulk quantities and as customized solutions.

Specifications

  • Available sizes: 100 mL, 500 mL, 1 L, bulk
  • Format: Ready-to-use (RTU)
  • Storage: 2-8⁰C in the dark
  • Stability: 4 years
  • Development time: 5-30 minutes
  • Stop solution: 0.2 M H₂SO₄
Request a sample Request
Questions, or buy this product? Contact us

ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
  • Reduces Water Waste
All about eco-tek

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